English
norskBlar i forfatter "Kaminski, Clemens F."
Viser treff 1-11 av 11
-
Concepts for structured illumination microscopy with extended axial resolution through mirrored illumination
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-03-20)Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical sectioning and to double the resolution limit both laterally and axially, but even with this the axial resolution is still worse than the lateral resolution of ... -
Label-free nanoscopy enabled by coherent imaging with photonic waveguides
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-29)In this project it was found that Fourier ptychographic microscopy can be improved far beyond its conventional limits via waveguide-based optical systems. Extensive in silico studies showed that images obtained on highrefractive index material waveguide chips in conjunction with hyperspectral illumination light and finely designed waveguide geometries can be combined via a modified phase-retrieval ... -
On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-12-10)Ribosome assembly occurs mainly in the nucleolus, yet recent studies have revealed robust enrichment and translation of mRNAs encoding many ribosomal proteins (RPs) in axons, far away from neuronal cell bodies. Here, we report a physical and functional interaction between locally synthesized RPs and ribosomes in the axon. We show that axonal RP translation is regulated through a sequence motif, ... -
OptiJ: Open-source optical projection tomography of large organ samples
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-10-30)The three-dimensional imaging of mesoscopic samples with Optical Projection Tomography (OPT) has become a powerful tool for biomedical phenotyping studies. OPT uses visible light to visualize the 3D morphology of large transparent samples. To enable a wider application of OPT, we present OptiJ, a low-cost, fully open-source OPT system capable of imaging large transparent specimens up to 13 mm tall ... -
A Protocol for Single-Molecule Translation Imaging in Xenopus Retinal Ganglion Cells
(Chapter; Bokkapittel, 2020-04-29)Single-molecule translation imaging (SMTI) is a straightforward technique for the direct quantification of local protein synthesis. The protein of interest is fused to a fast-folding and fast-bleaching fluorescent protein, allowing one to monitor the appearance of individual fluorescence events after photobleaching of pre-existing proteins in the cell under investigation. The translation of individual ... -
Single-Molecule Sizing through Nanocavity Confinement
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-02-24): An approach relying on nanocavity confinement is developed in this paper for the sizing of nanoscale particles and single biomolecules in solution. The approach, termed nanocavity diffusional sizing (NDS), measures particle residence times within nanofluidic cavities to determine their hydrodynamic radii. Using theoretical modeling and simulations, we show that the residence time of particles ... -
Super-condenser enables labelfree nanoscopy
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-08-22)Labelfree nanoscopy encompasses optical imaging with resolution in the 100 nm range using visible wavelengths. Here, we present a labelfree nanoscopy method that combines coherent imaging techniques with waveguide microscopy to realize a <i>super-condenser</i> featuring maximally inclined coherent darkfield illumination with artificially stretched wave vectors due to large refractive indices of the ... -
Super-condenser enables labelfree nanoscopy
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-08-22)Labelfree nanoscopy encompasses optical imaging with resolution in the 100 nm range using visible wavelengths. Here, we present a labelfree nanoscopy method that combines coherent imaging techniques with waveguide microscopy to realize a <i>super-condenser</i> featuring maximally inclined coherent darkfield illumination with artificially stretched wave vectors due to large refractive indices of the ... -
Superresolving the kidney – a practical comparison of fluorescence nanoscopy of the glomerular filtration barrier
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-12-05)Immunofluorescence microscopy is routinely used in the diagnosis of and research on renal impairments. However, this highly specific technique is restricted in its maximum resolution to about 250 nm in the lateral and 700 nm in the axial directions and thus not sufficient to investigate the fine subcellular structure of the kidney’s glomerular filtration barrier. In contrast, electron microscopy ... -
A waveguide imaging platform for live-cell TIRF imaging of neurons over large fields of view
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-02-17)Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been demonstrated for short‐term live‐imaging of robust cell types [3‐5], but not yet for delicate primary neurons nor over extended periods of time. Here, we present a waveguide‐based ... -
α-Synuclein fibril and synaptic vesicle interactions lead to vesicle destruction and increased lipid-associated fibril uptake into iPSC-derived neurons
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-05-15)Monomeric alpha-synuclein (aSyn) is a well characterised protein that importantly binds to lipids. aSyn monomers assemble into amyloid fibrils which are localised to lipids and organelles in insoluble structures found in Parkinson’s disease patient’s brains. Previous work to address pathological aSyn-lipid interactions has focused on using synthetic lipid membranes, which lack the complexity of ...
